IGF1 ELISA Kit

Catalog No. ABIN5523429

Human IGF1 ELISA Kit Colorimetric assay for quantification of Human IGF1.

Quick Overview for IGF1 ELISA Kit (ABIN5523429)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details IGF1 ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Purpose: For quantitative detection of IGF-1 in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of IGF-1. No significant cross-reactivity or interference between IGF-1 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IGF-1 and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 37.5 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF-1

Background: IGF-1/IGF1/IGFI/IGF-I/IGF1A/IGF-IA/IGF-IB/MGF/Somatomedin C

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation